The vote was to adopt the war powers resolution, which requires Trump to withdraw US forces or seek congressional approval for the conflict. BBC reported.
It is unclear how much legal force this House measure would have. The White House has dismissed the merits of the resolution, saying it is an unconstitutional attempt to restrict presidential power.
But its passing is significant because it adds to the pressure on the White House to find an end to the Iran War, as petrol prices have spiked and public opposition to the war has increased.
This was the fourth attempt by the House to rein in Trump's war powers. The Senate advanced a similar resolution in May but has yet to hold a full floor vote.
measure considered on Wednesday was a concurrent resolution. If it is also passed by the Republican-controlled US Senate, it would not require the president's signature, though it could be subject to a legal challenge.
The vote on Wednesday marked the latest sign of division within Trump's Republican Party, coming just days after a revolt by conservatives in Congress led his administration to pull back plans for a $1.8b "anti-weaponization" fund for political allies.
In the House, Republicans Thomas Massie, Brian Fitzpatrick, Tom Barrett, and Warren Davidson joined a united front from Democrats to pass Wednesday's resolution. Democrat Jared Golden of Maine, who had previously voted against similar measures, gave his support this time.
"Congress alone declares war, that's something certainly we need to be protective of," Barrett, a Republican from Michigan, said. Asked if he was worried about retribution from Trump for his vote, Barrett said: "I vote my conscience for what I think is right and willing to accept that."
