Four people were killed and 19 injured in the northeastern city of Kharkiv, while one person died and 16 were hurt in the capital Kyiv.
Ukrainian President Volodymyr Zelensky described the raid on Kyiv as "one of the most massive ballistic attacks" on the capital since the start of Russia's full-scale invasion in 2022. BBC reported.
Meanwhile Ukraine has continued its assault on Russian sites. Three Russian oil tankers in the Black Sea were hit, as well as facilities in the southwestern Stavropol region, Zelensky said.
A number of areas in the Kyiv region were hit overnight. The Ukrainian military said air defences in the capital had shot down 18 out of 41 missiles. The systems also intercepted 108 drones.
Kharkiv regional head Oleh Syniehubov said a postal terminal in the city suburbs had been attacked, with four men aged between 24 and 62 killed. Several of the injured were in a serious condition.
